The video, posted last Sunday by content creator Matt Watson, illustrated how YouTube's algorithm would apparently route users from regular videos to content featuring children within just a few clicks.
Despite growing pressure on Google and YouTube to crack down on offensive content, the firms' advertising revenues continue to grow.
Advertisers such as Nestle and Epic Games contacted by a news reporter who replied they will surely take action to stop their advertisements from displaying in front of Anti-Vax video or channels.
According to BuzzFeed, which first reported the news, channels that will likely be cut off from monetization include VAXXED TV, LarryCook333 and iHealthTube.
This cycle of back-and-forth between advertisers and YouTube is likely to keep repeating on open platforms because of the volume of uploaded content on the platform.
Remember YouTube's parody problem past year?
Family of IS teen to fight UK decision to strip citizenship
When London teenager Shamima Begum fled Britain with two other schoolgirls in 2015 to join the Islamic State, it shocked a nation. The Home Secretary wrote to Begum to inform her of her loss of citizenship to which she said she was "disappointed".
In a statement, a YouTube spokesperson said, "Any content-including comments-that endangers minors is abhorrent, and we have clear policies prohibiting this on YouTube".
DeFranco also said that the problem is not only with YouTube.
Watson criticized YouTube for monetizing these videos: he called out major brands, including McDonald's, Lysol, Disney, Reese's, Dodge Ram, and Maybelline, among many others. That includes videos promoting bogus miracle cures for serious illnesses, claiming the Earth is flat, or making "blatantly false claims" about historical events like the 9/11 terrorist attacks, according to YouTube.
The company also disabled comments on millions of videos featuring minors. Other companies like Peloton are looking into this situation as well. On Tuesday, the Wall Street Journal reported that Pinterest had essentially broken search results for "vaccines"; If someone searched for vaccines on Pinterest, the platform simply wouldn't deliver results.
AT&T Inc. withdrew all of its advertising from Alphabet Inc.'s YouTube for the second time in the past couple of years, after a magazine report revealed that the platform had been displaying ads next to videos, which contained child exploiting contents.
US Congressman Adam Schiff sent a letter to Google CEO Sundar Pichai and Facebook CEO Mark Zuckerburg last week expressing concern over the information on both companies' sites that "discourages parents from vaccinating their children, contributing to declining vaccination rates which could reverse progress made in tackling vaccine-preventable diseases".